BACT/ALERT® Bottle: Blood Culture Collection - Syringe Draw Method



This video describes the steps needed to collect and inoculate BACT/ALERT blood culture bottles using the Syringe draw procedure.

Steps Included:

  1. Have all the necessary supplies within reach
  2. Identify the patient
  3. Select and clean the venipuncture site
  4. Inspect bottles, mark the fill volumes, remove the flip-top and clean the septum
  5. Assemble the syringe and safety needle device
  6. Perform the venipuncture
  7. Aspirate the appropriate amount of blood.
  8. Place a gauze over the puncture site and withdraw the needle from the vein. Activate the safety device, remove the needle from the syringe and discard it into a sharps container.
  9. Attach the safety transfer device to the syringe.
  10. Inoculate the anaerobic bottle first, then the aerobic and finally any additional tubes if required.
  11. Discard the transfer device and syringe into a sharps container.
  12. Invert the bottles 3-5 times
  13. Label with patient information
  14. Bandage patient arm
  15. Place bottles and tubes into a biohazard bag and transport to the lab.

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